Publications
A modular platform for engineering function of natural and synthetic biomolecular condensates
Poly(A)-binding protein is an ataxin-2 chaperone that regulates biomolecular condensates.
Boeynaems S, Dorone Y, Zhuang Y, Shabardina V, Huang G, Marian A, Kim G, Sanyal A, Şen NE, Griffith D, Docampo R, Lasker K , Ruiz-Trillo I, Auburger G, Holehouse AS, Kabashi E, Lin Y, Gitler AD. Mol Cell. 2023 Jun 15;83(12):2020-2034.e6. DOI: 10.1016/j.molcel.2023.05.025.
Machine-learning-based methods to generate conformational ensembles of disordered proteins.
Taneja I, Lasker K. Biophys J. 2024 Jan 2;123(1):101-113. DOI: 10.1016/j.bpj.2023.12.001.
Aberrant phase separation is a common killing strategy of positively charged peptides in biology and human disease
Boeynaems S, Ma XR, Yeong V, Ginell GM, Chen JH, Blum JA, Nakayama L, Sanyal A, Briner A, Van Haver D, Pauwels J, Ekman A, Schmidt HB, Sundararajan K, Porta L, Lasker K, Larabell C, Hayashi MAF, Kundaje A, Impens F, Obermeyer A, Holehouse AS, Gitler AD. BioRxiv. 2023. DOI:10.1101/2023.03.09.531820
Integrative visualization of the molecular structure of a cellular microdomain.
Goodsell D, Lasker K.
Protein Sci. 2023. DOI:10.1002/pro.4577
The material properties of a bacterial-derived biomolecular condensate tune biological function in natural and synthetic systems
Lasker K*, Boeynaems S*, Lam V, Scholl D, Stainton E, Briner A, Jacquemyn M, Daelemans D, Deniz A, Villa E, Holehouse AS, Gitler A, Shapiro L.
Nat Commun
. 2022. DOI:10.1038/s41467-022-33221-z
Generative modeling of short, disordered proteins with homogeneous sequence composition
Taneja I, Lasker K*.
BioRxiv
. 2022. DOI:10.1101/2022.11.11.516154
Poly(A)-binding protein is an ataxin-2 chaperone that emulsifies biomolecular condensates
Boeynaems S, Dorone Y, Marian A, Shabardina V, Huang G, Kim G, Sanyal A, Şen N-E, Docampo R, Ruiz-Trillo I, Lasker K, Auburger G, Kabashi E, Gitler AD.
BioRxiv. 2021. DOI: 10.1101/2021.08.23.457426
ATAC-seq reveals megabase-scale domains of a bacterial nucleoid
Melfi MD, Lasker K, Zhou X, Shapiro L
BioRxiv. 2021. DOI:10.1101/2021.01.09.426053
The In Situ Structure of Parkinson's Disease-Linked LRRK2.
Watanabe R, Buschauer R, Böhning J, Audagnotto M, Lasker K, Lu TW, Boassa D, Taylor S, Villa E.
Cell. 2020; 182 (6), 1508-1518. PMID: 32783917
Selective sequestration of signalling proteins in a membraneless organelle reinforces the spatial regulation of asymmetry in Caulobacter crescentus.
Lasker K*, von Diezmann L*, Zhou X, Ahrens DG, Mann TH, Moerner WE, Shapiro L.
Nat Microbiol. 2020; 5(3):418-429. PMID: 31959967
A Localized Complex of Two Protein Oligomers Controls the Orientation of Cell Polarity.
Perez AM, Mann TH, Lasker K, Ahrens DG, Eckart MR, Shapiro L.
mBio. 2017; 8(1):e02238-16. PMID: 28246363
An intracellular compass spatially coordinates cell cycle modules in Caulobacter crescentus.
Lasker K*, Mann TH*, Shapiro L.
Curr Opin Microbiol. 2016; 33:131-139. PMID: 27517351
Cell cycle progression in Caulobacter requires a nucleoid-associated protein with high AT sequence recognition.
Ricci DP, Melfi MD, Lasker K, Dill DL, McAdams HH, Shapiro L.
Proc Natl Acad Sci U S A. 2016;113(40):E5952-E5961. PMID: 27647925
CauloBrowser: A systems biology resource for Caulobacter crescentus.
Lasker K, Schrader JM, Men Y, Marshik T, Dill DL, McAdams HH, Shapiro L.
Nucleic Acids Res. 2016;44(D1):D640-5. PMID: 26476443
Peptide dimer structure in an Aβ(1-42) fibril visualized with cryo-EM.
Schmidt M, Rohou A, Lasker K, Yadav JK, Schiene-Fischer C, Fändrich M, Grigorieff N.
Proc Natl Acad Sci U S A. 2015;112(38):11858-63. PMID: 26351699
Topological models of heteromeric protein assemblies from mass spectrometry: application to the yeast eIF3:eIF5 complex.
Politis A, Schmidt C, Tjioe E, Sandercock AM, Lasker K, Gordiyenko Y, Russel D, Sali A, Robinson CV.
Chem Biol. 2015;22(1):117-28. PMID: 25544043
The coding and noncoding architecture of the Caulobacter crescentus genome.
Schrader JM, Zhou B, Li GW, Lasker K, Childers WS, Williams B, Long T, Crosson S, McAdams HH, Weissman JS, Shapiro L.
PLoS Genet. 2014;10(7):e1004463. PMID: 25078267
Finding the right fit: chiseling structures out of cryo-electron microscopy maps.
Villa E, Lasker K.
Curr Opin Struct Biol. 2014;25:118-25. PMID: 24814094
Modeling of proteins and their assemblies with the Integrative Modeling Platform.
Webb B, Lasker K, Velázquez-Muriel J, Schneidman-Duhovny D, Pellarin R, Bonomi M, Greenberg C, Raveh B, Tjioe E, Russel D, Sali A.
Methods Mol Biol. 2014;1091:277-95. PMID: 24203340
Conformational transitions in human translin enable nucleic acid binding.
Pérez-Cano L, Eliahoo E, Lasker K, Wolfson HJ, Glaser F, Manor H, Bernadó P, Fernández-Recio J.
Nucleic Acids Res. 2013;41(21):9956-66. PMID: 23980029
Assembly of macromolecular complexes by satisfaction of spatial restraints from electron microscopy images.
Velázquez-Muriel J, Lasker K, Russel D, Phillips J, Webb BM, Schneidman-Duhovny D, Sali A.
Proc Natl Acad Sci U S A. 2012;109(46):18821-6.PMID: 23112201
Macromolecular assembly structures by comparative modeling and electron microscopy.
Lasker K, Velázquez-Muriel JA, Webb BM, Yang Z, Ferrin TE, Sali A.
Methods Mol Biol. 2012;857:331-50. PMID: 22323229
Molecular architecture of the 26S proteasome holocomplex determined by an integrative approach.
Lasker K*, Förster F*, Bohn S, Walzthoeni T, Villa E, Unverdorben P, Beck F, Aebersold R, Sali A, Baumeister W.
Proc Natl Acad Sci U S A. 2012; PMID: 22307589
Putting the pieces together: integrative modeling platform software for structure determination of macromolecular assemblies.
Russel D, Lasker K, Webb B, Velázquez-Muriel J, Tjioe E, Schneidman-Duhovny D, Peterson B, Sali A.
PLoS Biol. 2012;10(1):e1001244. PMID: 22272186
The proteasomal subunit Rpn6 is a molecular clamp holding the core and regulatory subcomplexes together.
Pathare GR, Nagy I, Bohn S, Unverdorben P, Hubert A, Körner R, Nickell S, Lasker K, Sali A, Tamura T, Nishioka T, Förster F, Baumeister W, Bracher A.
Proc Natl Acad Sci U S A. 2012;109(1):149-54. PMID: 22187461
UCSF Chimera, MODELLER, and IMP: an integrated modeling system.
Yang Z, Lasker K, Schneidman-Duhovny D, Webb B, Huang CC, Pettersen EF, Goddard TD, Meng EC, Sali A, Ferrin TE.
J Struct Biol. 2012;179(3):269-78. PMID: 21963794
Modeling of proteins and their assemblies with the integrative modeling platform.
Webb B, Lasker K, Schneidman-Duhovny D, Tjioe E, Phillips J, Kim SJ, Velázquez-Muriel J, Russel D, Sali A.
Methods Mol Biol. 2011;781:377-97. PMID: 21877292
MultiFit: a web server for fitting multiple protein structures into their electron microscopy density map.
Tjioe E, Lasker K, Webb B, Wolfson HJ, Sali A.
Nucleic Acids Res. 2011;39:W167-70. PMID: 21715383
Determining macromolecular assembly structures by molecular docking and fitting into an electron density map.
Lasker K, Sali A, Wolfson HJ.
Proteins. 2010;78(15):3205-11. PMID: 20827723
Integrative structure modeling of macromolecular assemblies from proteomics data.
Lasker K, Phillips JL, Russel D, Velázquez-Muriel J, Schneidman-Duhovny D, Tjioe E, Webb B, Schlessinger A, Sali A.
Mol Cell Proteomics. 2010;9(8):1689-702. PMID: 20507923
Toward an integrated structural model of the 26S proteasome.
Förster F, Lasker K, Nickell S, Sali A, Baumeister W.
Mol Cell Proteomics. 2010;9(8):1666-77. PMID: 20467039
An atomic model AAA-ATPase/20S core particle sub-complex of the 26S proteasome.
Förster F, Lasker K, Beck F, Nickell S, Sali A, Baumeister W.
Biochem Biophys Res Commun. 2009;388(2):228-33. PMID: 19653995
Insights into the molecular architecture of the 26S proteasome.
Nickell S, Beck F, Scheres SH, Korinek A, Förster F, Lasker K, Mihalache O, Sun N, Nagy I, Sali A, Plitzko JM, Carazo JM, Mann M, Baumeister W.
Proc Natl Acad Sci U S A. 2009;106(29):11943-7. PMID: 19581588
Inferential optimization for simultaneous fitting of multiple components into a CryoEM map of their assembly.
Lasker K, Topf M, Sali A, Wolfson HJ.
J Mol Biol. 2009;388(1):180-94. PMID: 19233204
The structural dynamics of macromolecular processes.
Russel D, Lasker K, Phillips J, Schneidman-Duhovny D, Velázquez-Muriel JA, Sali A.
Curr Opin Cell Biol. 2009;21(1):97-108. PMID: 19223165
Protein structure fitting and refinement guided by cryo-EM density.
Topf M, Lasker K, Webb B, Wolfson H, Chiu W, Sali A.
Structure. 2008;16(2):295-307. PMID: 18275820
EMatch: discovery of high resolution structural homologues of protein domains in intermediate resolution cryo-EM maps.
Lasker K, Dror O, Shatsky M, Nussinov R, Wolfson HJ.
IEEE/ACM Trans Comput Biol Bioinform. 2007;4(1):28-39. PMID: 17277411
EMatch: an efficient method for aligning atomic resolution subunits into intermediate-resolution cryo-EM maps of large macromolecular assemblies.
Dror O, Lasker K, Nussinov R, Wolfson H.
Acta Crystallogr D Biol Crystallogr. 2007;63(Pt 1):42-9. PMID: 17164525